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Molecular Biology Techniques
Author: Walt Ream, Katharine G. Field
Publisher: Elsevier
ISBN: 0125839901
Pages: 234
Year: 1999
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This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter
Molecular Biology Techniques
Author: Sue Carson, Susan Carson, Heather Miller, D. Scott Witherow
Publisher: Academic Press
ISBN: 0123855446
Pages: 200
Year: 2012
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Manual of Molecular and Clinical Lab Immunology
Author: Barbara Detrick, Robert G. Hamilton, James D. Folds
Publisher: American Society for Microbiology Press
ISBN: 155581364X
Pages: 1340
Year: 2006-03-10
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Introduces new material that reflects the significant advances and developments in the field of clinical laboratory immunology. • Provides a comprehensive and practical approach to the procedures underlying clinical immunology testing. • Emphasizes molecular techniques used in the field of laboratory immunology. • Updates existing chapters and adds significant new material detailing molecular techniques used in the field. • Presents guidelines for selecting the best procedures for specific situations and discusses alternative procedures. • Covers aspects of immunology related disciplines such as allergy, autoimmune diseases, cancers, and transplantation immunology.
Molecular Genetics of Mycobacteria
Author: Graham F. Hatfull, William R. Jacobs, Jr.
Publisher: American Society for Microbiology Press
ISBN: 1555818838
Pages: 824
Year: 2014-09-10
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A comprehensive collection of perspectives by experts in mycobacterial molecular biology Mycobacterium tuberculosis causes one in four avoidable deaths in the developing world and kills more adults than malaria, AIDS, and all tropical diseases combined. Tuberculosis was named a global health emergency by the World Health Organization, a distinction no other disease has received. Although the study of mycobacterial genetics has expanded dramatically, with new investigations into mycobacterial growth, replication, metabolism, physiology, drug susceptibility, and virulence, most of the problems in tuberculosis control that existed in 2000 remain today. Advances in our understanding of mycobacterial genetics have been reflected in exciting recent developments. New diagnostic approaches can identify drug resistance within a few hours, promising new drugs are progressing through the pipeline and into the clinic, and a range of newly developed vaccines are being evaluated. It is an exciting time as the fruits of 30 years of intensive genetic investigation are finally beginning to emerge. Written by leading experts in the field, Molecular Genetics of Mycobacteria, Second Edition, • Discusses key areas of current research in mycobacterial genetics • Explains the genetics of the physiology, metabolism, and drug sensitivities of M. tuberculosis • Presents genetic approaches for manipulating M. tuberculosis This book is an invaluable resource for anyone interested in the molecular genetics and molecular biology of mycobacteria.
Computational Molecular Evolution
Author: Ziheng Yang
Publisher: Oxford University Press
ISBN: 0198566999
Pages: 357
Year: 2006-10-05
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This book describes the models, methods and algorithms that are most useful for analysing the ever-increasing supply of molecular sequence data, with a view to furthering our understanding of the evolution of genes and genomes.
Basic Techniques in Molecular Biology
Author: Stefan Surzycki
Publisher: Springer Science & Business Media
ISBN: 3642569684
Pages: 434
Year: 2012-12-06
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This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Recombinant DNA and Biotechnology
Author: Helen Kreuzer, Adrianne Massey
Publisher: Wiley-Blackwell
ISBN: 1555811760
Pages: 431
Year: 2001-01-01
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Written in clear, easy–to–understand language, this best–selling reference text and activities manual offers easy–to–implement lessons and classroom activities. Part I covers basic molecular biology, and Part II offers imaginative dry labs and wet labs that can be done by both college and precollege students. Part III is an innovative section addressing the social issues and public concerns of biotechnology. Extensive appendixes provide important background information on basic laboratory techniques and teaching resources, including overhead masters and templates. Adopted by numerous school systems, this unique book is an outgrowth of molecular biology and biotechnology teaching workshops. All of the exercises and lab activities have been extensively tested in the classroom by hundreds of high school teachers. Recombinant DNA and Biotechnology is designed to interest an international teaching audience and will enable all instructors to teach a reasonable amount of molecular biology and genetic engineering to students. No other book makes it so easy or compelling for teachers to incorporate the "new biology" into their biology, biological sciences, or general science curriculum. Recombinant DNA and Biotechnology: A Guide for Teachers will enable college and precollege teachers to plan and conduct an exciting and contemporary course on the basic principles, essential laboratory activities, and relevant social issues and concerns attendant to today′s molecular biology revolution. In addition to the complete text of the student edition, A Guide for Teachers also contains the answers to all discussion questions and extra background information and material on the scientific principles involved.
Methods for General and Molecular Microbiology
Author: C. A. Reddy, Terry J. Beveridge, John A. Breznak, George Marzluf
Publisher: American Society for Microbiology Press
ISBN: 1555812236
Pages: 1069
Year: 2007-08-17
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A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. • Provides a comprehensive compendium of methods used in general and molecular microbiology. • Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis. • Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions. • Presents methods for bacteria, archaea, and for the first time a section on mycology. • Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.
Clinical Laboratory Management
Author: Lynne S. Garcia
Publisher: American Society for Microbiology Press
ISBN: 1555817270
Pages: 1068
Year: 2013-11-15
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This totally revised second edition is a comprehensive volume presenting authoritative information on the management challenges facing today’s clinical laboratories. • Provides thorough coverage of management topics such as managerial leadership, personnel, business planning, information management, regulatory management, reimbursement, generation of revenue, and more. • Includes valuable administrative resources, including checklists, worksheets, forms, and online resources. • Serves as an essential resource for all clinical laboratories, from the physician’s office to hospital clinical labs to the largest commercial reference laboratories, providing practical information in the fields of medicine and healthcare, clinical pathology, and clinical laboratory management, for practitioners, managers, and individuals training to enter these fields.
Cellular and Molecular Biology of Filamentous Fungi
Author: Katherine Borkovich, Daniel J. Ebbole
Publisher: American Society for Microbiology Press
ISBN: 1555814735
Pages: 802
Year: 2010-02-02
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An ideal starting point for any research study of filamentous fungi. • Incorporates the latest findings from such disciplines as physiology, taxonomy, genomics, molecular biology and cell biology. • Begins with an historical perspective, cell morphology and taxonomy, and moves on to such topics as cell growth, development, metabolism, and pathogenesis. • Presents the full range of the fungal kingdom and covers important topics as saprophytes, pathogens and endophytes. • Serves as a recommended text for graduate and undergraduate students.
Soft Machines
Author: Richard Anthony Lewis Jones
Publisher: Oxford University Press
ISBN: 0198528558
Pages: 229
Year: 2004
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Enthusiasts look forward to a time when tiny machines reassemble matter and process information but is their vision realistic? 'Soft Machines' explains why the nanoworld is so different to the macro-world that we are all familar with and shows how it has more in common with biology than conventional engineering.
A Guinea Pig's History of Biology
Author: Jim Endersby
Publisher: Harvard University Press
ISBN: 0674027132
Pages: 499
Year: 2007
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Spotlights small and pivotal experiments that changed the course of science, including information on the study of guinea pigs, passion flowers, zebra fish, and viruses.
Abstract Algebra
Author: Thomas W. Judson
Publisher: Orthogonal Publishing L3c
ISBN: 1944325050
Pages: 434
Year: 2017-08-05
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Abstract Algebra: Theory and Applications is an open-source textbook that is designed to teach the principles and theory of abstract algebra to college juniors and seniors in a rigorous manner. Its strengths include a wide range of exercises, both computational and theoretical, plus many non-trivial applications. The first half of the book presents group theory, through the Sylow theorems, with enough material for a semester-long course. The second-half is suitable for a second semester and presents rings, integral domains, Boolean algebras, vector spaces, and fields, concluding with Galois Theory.
Current Protocols Essential Laboratory Techniques
Author: Sean R. Gallagher, Emily A. Wiley
Publisher: Current Protocols
ISBN: 047094241X
Pages: 664
Year: 2012-03-19
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The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.
Practical Immunology
Author: Frank C. Hay, Olwyn M. R. Westwood
Publisher: John Wiley & Sons
ISBN: 1405146737
Pages: 408
Year: 2008-04-15
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Practical Immunology is a basic text aimed at immunology students and researchers at all levels who need a comprehensive overview of the methodology of immunology. The rapid and startling innovations in immunology over the past two decades have their root in sound experimental practice and it has always been the aim of this book to educate researchers in the design and performance of complex techniques. It will appeal to students of immunlogy, graduate students embarking on bench science, or specialised immunologists who need to use an immunological technique outside their sphere of expertise. The definitive lab "bench book". A one stop resource. Techniques explained from first principles. Basic forms of apparatus described in detail. Totally revised with new user friendly layout to aid use in the lab. Includes useful hints and tips.